a375 ma1 human melanoma cells  (ATCC)

Journal: Developmental cell

Article Title: Macrophage-dependent cytoplasmic transfer during melanoma invasion in vivo

doi: 10.1016/j.devcel.2017.11.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam Cat#: ab13970 Mouse monoclonal anti-ZO-1 Life Technologies Cat#: 33-9100 Mouse monoclonal anti-MITF Abcam Cat#: ab12039 Rabbit polyclonal anti-dsRed Clontech Cat#: 632496 Bacterial and Virus Strains N/A N/A N/A Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Dako serum free block Agilent Cat#: X090930-2 DAPI Sigma Cat#: D9542 TissueTek Optimum Cutting Temperature Compound VWR Cat#: 25608-930 Prolong Gold antifade reagent Molecular Probes Cat#: P36934 Recombinant human M-CSF Peprotech Cat#: 300-25 eBioscience Fixable Viability Dye eFluor 780 Thermofisher Cat#: 65-0865-14 Lipopolysaccharide InvivoGen Cat#: R595 Collagenase Type I Worthington Biochemical Cat#: LS004194 DNase Worthington Biochemical Cat#: LS006361 Hyaluronidase Sigma Cat#: H3506 Red Blood Cell Lysis Buffer Biolegend Cat#: 420301 Critical Commercial Assays Incucyte zoom system Essen Bioscience Cat#: 4647 SP6 mMessage mMachine Life Technologies Cat#: AM1340 Lipofectamine 3000 Invitrogen Cat#: L3000001 Golden Gate TALEN and TAL effector kit Addgene Cat#: 1000000024 Deposited Data N/A N/A N/A Experimental Models: Cell Lines Human melanoma cells: A375P ATCC Cat#: CRL3224 Human melanoma cells: A375M1 ATCC Cat#: CRL3222 Human melanoma cells: WM266-4 Rockland Cat#: WM266-4-01-0001 Human melanoma cells: 1205Lu Coriell Institute Cat#: WC00058 Human melanoma cells: WM793 Coriell Institute Cat#: WC00062 Murine melanoma cells: B16F10 ATCC Cat#: CRL-6475 Human epithelial cells: MCF10A ATCC Cat#: CRL-10317 Murine intestinal cancer cells: MC-38 Kerafast Cat#: ENH204 MLV-based retroviral packaging cell line: PT67 ATCC Cat#: CRL-12284 Human melanoma cells: Mel-624 Laboratory of Randall Moon N/A Zebrafish melanoma cells: Zmel Laboratory of Richard White Heilman et al., 2015 .

Techniques: Virus, Recombinant, Blocking Assay, Red Blood Cell Lysis, Retroviral, Sequencing, Mutagenesis, Binding Assay, CRISPR, Plasmid Preparation, Software

Journal: Developmental cell

Article Title: Macrophage-dependent cytoplasmic transfer during melanoma invasion in vivo

doi: 10.1016/j.devcel.2017.11.003

Figure Lengend Snippet: (A, B) Schematic of zebrafish hindbrain ventricle transplantation and imaging strategy. (C) Confocal Z-projection of fixed larvae in which melanoma cells (GFP immunostaining, green) are dorsal to the choroid plexus, within the skin (ZO-1 immunostaining, red). Scale bar is 20 uM. See also Figure S1 and Movie S1. (D) Left: DIC image of anterior region of 6dpf zebrafish larva on left. Right: Fluorescence image of same larva showing transplanted tumor (arrow) and disseminated cells (arrowheads). Scale bar is 100 uM. (E) Quantification of tumor cell dissemination in vivo in which each point is a single experiment comprising of 20–40 larvae. n indicates total number of zebrafish scored. Grey boxes indicate matched pairs of primary tumor-derived cell lines (A375P, WM793), and cell lines derived from metastases of those parental tumors (A375M1, 1205Lu). One-way ANOVA (p<0.0001), followed by non-parametric unpaired t-test (p=0.05 (between A375P and A375M1), p=0.01 (between WM793 and 1205Lu)).

Article Snippet: Human melanoma cells: A375M1 , ATCC , Cat#: CRL3222.

Techniques: Transplantation Assay, Imaging, Immunostaining, Fluorescence, In Vivo, Derivative Assay

Journal: Developmental cell

Article Title: Macrophage-dependent cytoplasmic transfer during melanoma invasion in vivo

doi: 10.1016/j.devcel.2017.11.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human melanoma cells: A375M1 , ATCC , Cat#: CRL3222.

Techniques: Virus, Recombinant, Blocking Assay, Red Blood Cell Lysis, Retroviral, Sequencing, Mutagenesis, Binding Assay, CRISPR, Plasmid Preparation, Software